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Explore the Advantages of ACE C18-PFP:
a unique C18 bonded HPLC column
with the extra selectivity
of a pentafluorophenyl (PFP) phase
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Combines C18 and PFP mechanisms of separation to separate mixtures not possible with either phase alone
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Ultra inert, ultra high purity silica, for excellent peak shape & reproducibility
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Exceptional bonded phase stability for elevated temperature applications
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Ultra low bleed phase ensures UV and LC/MS compatibility
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Available in high throughput column dimensions
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| CLICK HERE for further details of the ACE C18-PFP brochure |
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| Why do I need another new C18 phase? |
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The use of an ultra pure, ultra inert silica has many recognised benefits including improved reproducibility, lifetime and chromatographic performance (particularly with basic molecules). However, since the ultra inert silica surface effectively no longer contributes to the separation, C18 columns manufactured with high purity silicas show near identical selectivity. It is therefore highly likely that a problem separation on one leading brand will not be significantly improved by changing to an alternate manufacturer's equivalent product.
For many years, experienced chromatographers have been seeking phases with the proven performance & reproducibility benefits shown by such leading C18 column brands, but which additionally provide the alternate selectivity required for their challenging applications.
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| How is ACE C18-PFP different? |
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C18 bonded phases currently dominate the HPLC market, with recent surveys indicating that they are still responsible for 50-60% of all HPLC columns sold. In recent years the use of PentaFluoroPhenyl (PFP) bonded phases has grown significantly due to the alternate selectivity they provide.
However, compared to C18 bonded phases, PFP phases have traditionally been compromised with reduced hydrophobicity, reduced stability and significant column bleed.
The ACE C18-PFP phase utilises a specially developed ligand combining a C18 chain with integral PFP functionality, resulting in a phase that maintains the hydrophobic, stability and low bleed characteristics of leading C18 phases, yet provides the multiple retention mechanisms of a PFP phase that are responsible for the unique selectivity of ACE C18-PFP.
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| When should I use ACE C18-PFP? |
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Due to their similar hydrophobic characteristics, ACE 18-PFP columns may be used for applications in which "standard" C18 columns would normally be considered. However, due to its integral pentafluorophenyl functionality, ACE C18-PFP is additionally recommended for separations that involve halogenated aromatic compounds, regioisomers and those analytes with differing shape constraints.
As the applications contained within the ACE C18-PFP brochure demonstrate, ACE C18-PFP can be used to improve separations that are proving problematic on C18 columns. The unique ACE C18-PFP phase provides an alternate selectivity to C18 columns, but remains a valid selection for methods in which C18 bonded columns are specified. In many instances, the same evaluation conditions that prove unsuitable for the C18 column prove suitable for the C18-PFP column, avoiding the need for lengthy method redevelopment.
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| Please contact your local distributor, see our coordinating UK distributor's website or contact ACT for further details |
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